Morini MO2 Clutch Cable Replacement

The right kind of clutch cable for an m02 morini, and the clever little clip that actually engages the clutch.

So I snapped the cable off the clutch lever of the Pilot the other day, and as a result, I went to San Fransisco to buy a new clutch cable (not really for that, but I did go to SF and get a clutch cable).  When I got home, I discovered two things:

  1. The people who designed the bike were not stupid
  2. I bought the wrong kind of cable!

The wrong kind of nubbin!

Grr.  The extra little bit above the very end nub prevents it from being able to be pulled, since the bottom nub makes the clip coming out of the engine rub against the guide.  Unfortunately, I had not removed the guide all the way and really given the system a peek until after I had bought the new cable.  Fortunately, a bike shifter cable is the perfect size, looks to be pretty strong, and is available in abundance in Somerville.  After picking one up, I managed to rig it up so that it can sort of engage the clutch.  It is tricky to get it engaged, but for the time being, yay testing!

DIYBIO – FBI Outreach Conference, San Fransisco

Actually, we were in Walnut Creek, but it is close enough to call it San Fransisco!

As it turns out, the FBI and other defense organizations (Hello, DTRA) are pretty interested in DIYBIO.  Coming away from the conference, it seems like the FBI is  interested in exactly what you would expect: preventing bad guys (nefarious actors!) from doing Bad Things.  The Defense Threat Reduction Agency on the other hand, is interested in buying technology from people who start in “garages”, or DIY environments, to use for defense work.

The room was pretty full! I didn’t know there were this many diybiologists!  (some of these people are FBI agents/wmd coordinators.  Hard to tell the difference in this photo)

The main focus of the conference was on the interaction between law enforcement and DIY biologists.  It seems to be that the FBI is not concerned with DIY biologists, and that the FBI certainly does not view the DIYBIO “movement” as a threat.  The position of the Bureau is that local DIYBIO folks should get a hold of their local WMD coordinator,  It was also reassuring to know that the FBI hires PHD biologists and a lot of scientists to work in their WMD department- it would be nice if policy makers were just as well informed.

There was also a good discussion about the media- it turns out that both the FBI and DIYBIO folks both tend to kind of dislike the media, because as one attendee put it “They overestimate our abilities, and underestimate our ethics”.  There were some good talks given on how to engage the press in a a way that cannot be misconstrued, and how to do due diligence when someone wants to cover your space.  Rachel had an anecdote from when the BBC approached them to do a piece on the DIYBIO activities at MADLAB/MCR:

The approach that we got. we are interested in debate, is't that lovely, PCR machines, exclamation points. This is what we read: we're going to do a piece on bioterror and flu virus research. And we knew that, we knew that we were going to be portrayed as extreme. We're the only group that can kind of say these things, we weren't the right people, but we were going to be their people anyway, and it was. This is what showed up in the BBC website.. "growing concern about DIYbio.. FBI, oh there you are". Biological threat, all in the same sentence.
( quote from transcript typed by Bryan Bishop )

I thought it was very useful that we had Dan Grushkin, Rachel Turner, and Sascha Karburg -who have both done quite a bit of journalism- to tell us how the journalism works.  It is important to have both sides of the story to really understand what is going on, so DIYers can engage the press more tactfully.

Speaking of Sascha, we got to enjoy his documentary on DIYBIO at the end of the first day.  After a few years in the making, it looked pretty awesome!  I didn’t understand what they were saying most of the time, as it was in German, but the images definitely told a story.

I think that the highlight of the conference was finally seeing who was out there, and what they were up to.  If you want, you can read transcripts here, courtesey of Brian Bishop.

countries from left to right:
USA, The Netherlands (behind the benches), Finland, Denmark, Germany, Turkey

The last day we all went down to Biocurious to play with some DNA.  Biocurious walked everyone through the basic procedure for a chemical transformation, but the real highlight here was working with people from other places, and actually building a plasmid with the Genomikon kit.

Overall it was fun to meet everyone, and exciting to see what the rest of the diybio folks are up to.  I think finally meeting the European counterparts helped bring the community together.  And it was certainly good to learn that the FBI won’t be knocking on our door any time soon.

FBI-DIYBIO Outreach Workshop

I have been invited to California to the FBI-DIYBIO outreach workshop.  Day one is tomorrow.  As I sit here slurping at the last of of my large java-chip frappacino (with whipped cream) at Bryant and Mariposa, I have to say that I am pretty psyched to see Biocurious, and meet all the other DIYBIO folks to compare notes.  I will be posting my notes here on what happens!

Visited the Treatland.tv In Real Life.

is this treatland?

I am in San Fransisco for reasons which are better covered in a later post, but it was a stroke of luck because I needed some parts from treatland, but I couldn’t buy them online.  So I just walked over to 3077 17th street in San Francisco…and proceeded to pace back and forth for a bit.

Not treatland. SRS BZNSS HONDAS

You would think that this was treatland, but it wasn’t.  It is apparently a honda bike shop, and they give you a weird look when you walk by because they are SRS BUSINESS.  They also have a nice sign that says something about not working on mopeds, not lending tools and to stay out of the shop.  I thought to myself, surely this couldn’t be treatland…where are the treats?

Treats is here!  In the building marked SH FRANK AND CO.

As I mustered up the courage to go ask them for moped parts, I saw someone pull into a garage across the street on a Derbi.  Ah, I thought, this must be the treatland.  And so it was!  I got a knarp, a clutch cable, a tail light, and a bunch of jets.  Sweet!

And I even saw this sign…

Treat Street

Malaguti Update: 6/7/2012

Got the ‘guti running!  Good stuff.  Vroom vroom.  Or maybe like, vrrrr….

I hope I look this good when I turn 40!

There are still a few slight issues, but I got it to run by adjusting the spark gap with some .4 mm metal sheeting, which I found at sprout.  I think it is for weather proofing.  The other suggestion for a feeler gauge stand in that I got was a cereal box, so the piece of metal seemed like a decent option, given that it measured .34-.41 mm on some digital callipers, and the gap should be .35-.4 mm for a Morini MO2 (M02?) engine.

A picture showing the electrical SNAFU

Outstanding issues are now that the electrical system is in a small SNAFU, because I hard-wired EVERYTHING, disconnected the killswitch, the tail light is broken, and the lighting coil is not tested or installed.  Also, the carb needs to be re-jetted and the idle adjusted, since it was drilled out by the previous owner.  And the last thing is that the clutch cable broke!  Oh no!  Better go to treats.  Oh, yea and the pipe is clogged.

Whats missing? The tail light is missing…

Other than all that, I got it moved to the new workspace okay by yanking the clutch cable real hard with my left hand, giving it gas with my right hand, and peddling really really hard.  I got all the way down one or two blocks before I ran into a one way street in the other direction and the engine died again.  Bugger.

Malaguti Pilot: A New Project

The ” ‘guti “, a malaguti pilot from the 70’s

I picked this moped up from kHz Garage in Allston- not running in its current condition, but with a few donor electrical and fuel related parts, it had started up, according to the previous owners.  I pedal/push/pulled it all the way from allston, up through Harvard and porter square to sprout to sit down with it for a few days to get it repaired.  It was a long and very exhausting trip, but the next day I got to look at some of the things that were not functioning.

Something is missing here…

Something is missing here.  Some kind of tube to convey liquid fuel (gasoline+2 stroke premix) to the carb.  Ah, a fuel line and fuel filter are in order.  In short order, the magic of 3/16 in (4mm, I believe) fuel line and a cheap, $5 filter fixed this problem.

Something is missing here too! What could it be…?

I got a new spark plug boot as part of the bike, but I needed some B6HS spark plugs for the Franco Morini Motori MO2 (motor).  So I got some and installed one.

fuel line and filter routed around stash box

 

Here you can see the fuel line and filter mounted around the stash box, near the side panels of the body.  The stash box is pretty sweet- it looks like a good place to stash some extra fuel line, a fresh plug, or maybe some small tools.  In the background you can see the spark plug mounted at a funky angle.  Way Rad!

Well, that is all for now.  There is no spark since I still have not wired the magneto yet- once I do that it is time to test the whole system!

How Do I Get Started In DIYBIO?

A lot of people have been asking “How do I get started in DIYBIO?”.  The answer is not easy.  Biology is a broad field, ranging from studying entire ecosystems, to the chemicals that allow life to continue.  But I have done my fair share of DIY molecular biology, and I have begun to write up protocols and reviews of equiptment- which I will begin to share on this site on the DIYBIO protocols page, and on BOSSLAB.

Enjoy!  More posts on how to get started to come.

Inspiring People: Cellfyre, Andreas Sturm, and Chris Templeman

Every now and then I run into or chat with people who are really excited about DIYBIO, and it is really these people who get me excited about what I do at BOSSLAB.  I just wanted to give a shutout to a few people who have recently been awesome:

Cellfyre is from Oregon, and intends to start a DIYBIO group out there!  According to her twitter, “Everybody is handed a coupon for scientific knowledge at birth. Many have forgotten to redeem theirs. To create awareness I advocate #DIYBio, #STEM, and #PLoS“.  She came down to BOSSLAB (last week? hard to tell with finals going on) and we talked about what it is like to run a DIYBIO lab, sourcing equipment, and getting people excited.  You can (and should) check out here twitter here!  If you are in Oregon, tweet at her!

Andreas Sturm is a student who works somewhere in Europe (I am unsure of where), and is very ambitious in the development of hardware.  His do it yourself bio-hardware blog should be up soon- I will definitely share it here once it is up!  Since bio-engineering of any kind is very taboo- to the point of being illegal- he has to work closely with his university to do even transformations!  But he still persists in being curious and hacking up useful tools.

Chris Templeman is an engineer and presumably owns Templeman Automation in Somerville.  It turns out he was the driving force behind the sub $200 (if i recall correctly) PCR machine that was released by cofactor bio a while back.  He is smart, excited about biology, so keep an eye out for him.  He is also behind a pretty sweet multitouch computing table kickstarter, here.

Gene Cloning: Successful!

Today, amid the project crises going on for my various classes, I got some very good and very exciting news:  my engineered microbial systems project seems to be going well!

A little background on the project and team:

Our team consists of three people:  Neal Singer (MechE), Jea Young Park (E:Bio) and myself (MechE).  Our professor is Jean Huang, who is awesome and exceedingly good at juggling multiple projects and inspiring us to do cool things.  The goal of our team was to (in the span of a month), clone a gene from p. Atlantica into e. Coli.  It sounds like it should be simple and routine, but it is actually quite a process.  The good news is it seems to have worked!

How do we know it worked?  Check out these gels:

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The “top” of the gel is side with the wells closest to the edge.  From left to right, we ran the PCR products from samples of plasmid from transformants in wells 1-9, and the 10th one was a positive control of PCR product of genomic DNA.  The idea is that if the plasmid contains the gene, we would see a band near the positive control which definitely contains a copy of the gene.  And the result is that we do!  This means that when we ligated the plasmid and the newly cloned gene, some of the copies re-circularized without the gene, and some of the copies incorporated the new gene, and some of those copies of the gene ended up in bacteria that we have growing in the lab.

Sweeeeeeeeeet.

Genomes, Environments and Traits Confrence!

Jason Bobe moderates a discussion with George Church and Geraldine Hamilton about personalized medicine microfluidic devices

Today I attended the Genomes Environments and Traits Conference.  It was awesome!  There were talks on all manner of technical breakthroughs from faster and cheaper sequencing, to single-cell sequencing (WITH 3D protein/DNA localization on the intracellular level!!!), to hackable drug delivery kits for 3rd world countries.

The more exciting part for me personally was running into all kinds of DIYBiologists.  It was awesome to finally meet them in person!  Ellen Jorgensen from GeneSpace was there, as well as Joseph Jackson from BioCurious, and (obviously) Jason Bobe from the Personal Genome Project.  There were also some people from the BOSSLAB group there (woo! not sure if they want to be mentioned by name).  I even found somebody from Olins’ neighbor college, Wellesley, and I spotted at least one Babson Jacket in the crowd.

Anyways, this conference got me more excited about biology and science, and this summer at BOSSLAB.