GelGreen: As Good As Advertised

Check out the bands on the right! Its a DNA ladder. Its pretty smiley because I was running at high amperage just to test the new stain.

I ordered some GelGreen from Phenix research the other day, because they sell it in .1 ml quantities, unlike the other suppliers in the US, and because they will just take my money and ship me things without a probing questionnaire or having to call or email them (again, unlike the other US distributors).  The .1 ml quantity also sells at the same cost/unit as the .5 ml quantity!  Combined with my ability to illuminate it with some cheepo blue LEDs from ebay, incredibly low toxicity and cell permeability, and stability at room temperature in water, I would recommend it as an alternative to Carolina’s “Carolina Blu” DNA stain, which is awful.  It also makes a fine alternative to ethidium bromide if you don’t want to have hazardous chemicals around, or if you don’t want to have to buy expensive UV gel documentation equipment.
I guess my only question for Biotum is…why the heck is it called GelGreen?  Nothing about it is green, as far as I can tell.  Its Fluorescence is really more of a yellowy color, according to the datasheet.

Click here to find out more about GelGreen

And Here to buy GelGreen from Phenix Research

Bioethics: Don’t Ship Invasive Species

A few days ago I naively asked Gulf Coast Ecosystems if they would ship me some Bryopsis cultures.  They noted that they do not normally stock it because it is highly invasive.  At first I thought they meant that it was highly invasive in fishtanks, but then it dawned on me that they meant that it was highly invasive to local waterways.  I certainly do not want to be responsible for messing up the local ecosystem, but I may have inadvertently done that had I not been informed by a responsible seller.  +1 Gulf Coast Ecosystems, +1.