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some sparks!2020-10-29T01:06:20+00:00weekly0.6https://aylo6061.com/2020/09/27/little-pond-big-dive/2020-09-27T18:58:43+00:00monthlyhttps://aylo6061.com/2017/04/27/just-in-time-implementation-of-travel-ukulele/https://aylo6061.com/wp-content/uploads/2017/06/img_6238.jpgIMG_6238https://aylo6061.com/wp-content/uploads/2017/06/img_6251.jpgIMG_6251https://aylo6061.com/wp-content/uploads/2017/06/img_6260.jpgIMG_6260https://aylo6061.com/wp-content/uploads/2017/06/img_6228.jpgIMG_62282019-11-27T22:23:36+00:00monthlyhttps://aylo6061.com/2019/10/14/oxygen-rebreather-design-and-fabrication/https://aylo6061.com/wp-content/uploads/2019/10/inspiration.pnginspiration2022-09-22T15:03:49+00:00monthlyhttps://aylo6061.com/2017/03/24/withings-activite-pop-teardown/https://aylo6061.com/wp-content/uploads/2017/03/img_0062.jpgIMG_0062https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_195146140.jpgIMG_20170302_195146140https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_180533889.jpgIMG_20170302_180533889https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_172704294.jpgIMG_20170302_172704294https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_1726172881.jpgIMG_20170302_172617288https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_172617288.jpgIMG_20170302_172617288https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_172454752.jpgIMG_20170302_172454752https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_171908229.jpgIMG_20170302_171908229https://aylo6061.com/wp-content/uploads/2017/03/img_20170302_171634254.jpgIMG_20170302_171634254https://aylo6061.com/wp-content/uploads/2017/03/img_0147-e1488519014449.jpgimg_01472019-11-17T21:01:26+00:00monthlyhttps://aylo6061.com/2017/04/03/withings-pop-activite-electronic-analysis/https://aylo6061.com/wp-content/uploads/2017/03/img_20170306_195524907.jpgIMG_20170306_195524907https://aylo6061.com/wp-content/uploads/2017/03/5x-press3.png5x 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white2016-07-23T12:32:38+00:00monthlyhttps://aylo6061.com/2016/07/23/gopro-viewfinder/https://aylo6061.com/wp-content/uploads/2016/07/eyefinder.jpgeyefinderhttps://aylo6061.com/wp-content/uploads/2016/07/img_20160720_165129199.jpgIMG_20160720_165129199https://aylo6061.com/wp-content/uploads/2016/07/img_20160720_165046844.jpgIMG_20160720_1650468442016-07-23T04:34:21+00:00monthlyhttps://aylo6061.com/2016/07/22/the-best-coffee-in-washington-d-c/2016-07-23T03:40:21+00:00monthlyhttps://aylo6061.com/2016/07/22/filter-cafe/https://aylo6061.com/wp-content/uploads/2016/07/gopr5661.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5660.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5657.jpgDCIM104GOPRODCIM104GOPRO2016-07-23T03:37:48+00:00monthlyhttps://aylo6061.com/2016/07/22/dolcezza/2016-07-23T03:37:33+00:00monthlyhttps://aylo6061.com/2016/07/22/northside-social/https://aylo6061.com/wp-content/uploads/2016/07/gopr5682.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5680.jpgDCIM104GOPRODCIM104GOPRO2016-07-23T03:37:21+00:00monthlyhttps://aylo6061.com/2016/07/22/slipstream/https://aylo6061.com/wp-content/uploads/2016/07/g0275926.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0255912.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0245893.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0205853.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0165822.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0155803.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0145793.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0135783.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0125772.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/g0105752.jpgDCIM104GOPRODCIM104GOPRO2016-07-23T03:37:01+00:00monthlyhttps://aylo6061.com/2016/07/22/compass-coffee/2016-07-23T03:36:44+00:00monthlyhttps://aylo6061.com/2016/07/22/chinatown-coffee-company/2016-07-23T03:36:26+00:00monthlyhttps://aylo6061.com/2016/07/22/peregrine-coffee/https://aylo6061.com/wp-content/uploads/2016/07/gopr5652.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5651.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5650.jpgDCIM104GOPRODCIM104GOPRO2016-07-23T03:36:11+00:00monthlyhttps://aylo6061.com/2016/07/22/baked-and-wired/https://aylo6061.com/wp-content/uploads/2016/07/gopr5678.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5677.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5675.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5673.jpgDCIM104GOPRODCIM104GOPROhttps://aylo6061.com/wp-content/uploads/2016/07/gopr5670.jpgDCIM104GOPRODCIM104GOPRO2016-07-23T03:35:52+00:00monthlyhttps://aylo6061.com/2016/07/22/la-colombe/2016-07-23T03:35:28+00:00monthlyhttps://aylo6061.com/2011/09/26/the-time-is-now/2016-07-23T03:32:05+00:00monthlyhttps://aylo6061.com/2011/09/26/attiny45-based-usbtinyisp-programmer/https://aylo6061.com/wp-content/uploads/2011/09/2011-09-26_17-08-07_547.jpgBreadboarded CircuitThis is how my breadboard looked when I was done. Note the diodes and the orientation of the chip. Specifically pin one is on the upper right hand corner.https://aylo6061.com/wp-content/uploads/2011/09/programmer_schematic.jpgProgrammer SchematicThis is a hand drawn, but clear representation of the circuit you want to build. Unlike the one on instructables, it doesent have (over 9000!) overlapping lines.https://aylo6061.com/wp-content/uploads/2011/09/2011-09-26_17-51-21_426.jpgUSB cables soldered to header pins5 Volts and Ground are soldered to one pair of pins, while D+ and D- are soldered to another pair2016-07-23T03:31:58+00:00monthlyhttps://aylo6061.com/2011/09/29/isolation-of-vibrio-fischeri-from-seafish/https://aylo6061.com/wp-content/uploads/2011/09/5944637912_5ea5100411_z.jpgZoomed in!Taken with long exposurehttps://aylo6061.com/wp-content/uploads/2011/09/5944599144_1809dc2ac5_z.jpgSquid in a box!Taken with long exposure2016-07-23T03:31:52+00:00monthlyhttps://aylo6061.com/2011/09/30/the-secret-knowledge/2016-07-23T03:31:47+00:00monthlyhttps://aylo6061.com/2011/09/30/the-secret-knowledge-vol-i-the-time-is-now/https://aylo6061.com/wp-content/uploads/2011/09/tsk_lab1_dia.jpgDiagrams from the handoutRed lines indicate errors or omissions in the printed document that was supplied to students2016-07-23T03:31:43+00:00monthlyhttps://aylo6061.com/2011/09/30/secret-knowledge-time-vol-ii-servos/https://aylo6061.com/wp-content/uploads/2011/09/555_2_schem1.pngSchematic for the servo controllerAgain, errors are marked with red Xs.2016-07-23T03:31:38+00:00monthlyhttps://aylo6061.com/2011/10/01/secret-knowledge-time-vol-iii-tiny-computer-time/2016-07-23T03:31:34+00:00monthlyhttps://aylo6061.com/2013/07/19/chorded-keyboard-why-build/2016-07-23T03:30:49+00:00monthlyhttps://aylo6061.com/2011/10/05/poeject-3-aka-the-v-usb/https://aylo6061.com/wp-content/uploads/2011/10/2011-10-08_20-08-03_543.jpgReally awfully breadboarded circuitBad breadboarding! Do not do!https://aylo6061.com/wp-content/uploads/2011/10/2011-10-08_20-07-20_975.jpg2011-10-08_20-07-20_975https://aylo6061.com/wp-content/uploads/2011/10/2011-10-08_20-00-10_562-e1318119347708.jpgLaser Turret!Laser Turret!https://aylo6061.com/wp-content/uploads/2011/10/pngbase64d9122eacb0011eac1.pngSecret Knolwedge ArduinoThe Secret Knowledge Arduino uses V-USB! the connections to Pin 1 are not needed for V-USB.https://aylo6061.com/wp-content/uploads/2011/10/pngbase64d9122eacb0011eac.pngSecret Knowledge Arduino runs on V-USB!This is the Secret Knowledge Arduino schematic, which runs V-USB. The important part is everything except for pin 1.2016-07-23T03:30:31+00:00monthlyhttps://aylo6061.com/2011/10/05/clock-project-inspiration-2/https://aylo6061.com/wp-content/uploads/2011/10/2011-10-05_19-55-46_406.jpgThe counter, sitting on the breadboard I am building the clock onhttps://aylo6061.com/wp-content/uploads/2011/10/2011-10-05_19-56-48_256.jpgThe box!2016-07-23T03:30:26+00:00monthlyhttps://aylo6061.com/2011/10/07/usb-control-transfer-sender/https://aylo6061.com/wp-content/uploads/2011/10/uglyinterfaceusb1.pngUgly interfaceUgly, and very horizontal interface2016-07-23T03:30:21+00:00monthlyhttps://aylo6061.com/2011/10/10/transformation/https://aylo6061.com/wp-content/uploads/2011/10/6060682378_6c9f223efc_b1.jpgSome plates of transformants...which ones are green?Some plates of transformants...which ones are green?https://aylo6061.com/wp-content/uploads/2011/10/6060144559_5db0c6cdf8_b.jpgTransformants under blue light with a green filterTransformants under blue light with a green filter. Green light is produced by the fluorescence, and then only green light is let through the filter.https://aylo6061.com/wp-content/uploads/2011/10/6060142073_8f633a418d_b.jpgTransformants under blue lightTransformants fluorescing under a blue LED.https://aylo6061.com/wp-content/uploads/2011/10/6060132419_6f685da983_b.jpgSatellite coloniesSatellite colonies around transformantshttps://aylo6061.com/wp-content/uploads/2011/10/6060682378_6c9f223efc_b.jpg6060682378_6c9f223efc_bhttps://aylo6061.com/wp-content/uploads/2011/10/pgreen.pngPlasmids!pGreen and a generic plasmid2016-07-23T03:30:15+00:00monthlyhttps://aylo6061.com/2012/02/27/diybio-aseptic-technique-as-learned-by-pouring-plates/https://aylo6061.com/wp-content/uploads/2012/02/2011-04-25_01-30-55_262.jpg2011-04-25_01-30-55_262As you can see, this is a Yeast Extract plate, streaked on 3/28/11 by me with Smooth' from a plate called T1 trop IIhttps://aylo6061.com/wp-content/uploads/2012/02/p1010501.jpgP1010501Ben carefully pours a platehttps://aylo6061.com/wp-content/uploads/2012/02/p1010498.jpgP1010498https://aylo6061.com/wp-content/uploads/2012/02/p10104891.jpgP1010489At this point, it will be hot and a liquid. Use autoclave gloves!https://aylo6061.com/wp-content/uploads/2012/02/p1010460.jpgP1010460Time to fire up the cooker!https://aylo6061.com/wp-content/uploads/2012/02/p10104581.jpgP1010458https://aylo6061.com/wp-content/uploads/2012/02/p10104571.jpgP1010457https://aylo6061.com/wp-content/uploads/2012/02/p1010456.jpgP1010456https://aylo6061.com/wp-content/uploads/2012/02/p1010454.jpgP1010454https://aylo6061.com/wp-content/uploads/2012/02/p1010453.jpgP1010453Read the instructions!2016-07-23T03:30:13+00:00monthlyhttps://aylo6061.com/2012/02/14/revobots-getting-ready/https://aylo6061.com/wp-content/uploads/2012/02/igp5120.jpg_IGP5120REVOBot 001https://aylo6061.com/wp-content/uploads/2012/02/igp5162.jpg_IGP5162Custom SMD Attiny45 USBISP programmer doing its job!2016-07-23T03:30:08+00:00monthlyhttps://aylo6061.com/2011/11/01/reverse-engineering-logitech-unifying-usb-protocol/2016-07-23T03:29:48+00:00monthlyhttps://aylo6061.com/2011/11/08/eee-pc-701-mods-derpyhooves/https://aylo6061.com/wp-content/uploads/2011/11/100_0523.jpgEEE 701 "DerpyHooves" MoBolarge yellow taped blob is the USB hub. Black labeled thing is the receiver. Card reader not pictured.https://aylo6061.com/wp-content/uploads/2011/11/100_0522.jpg701 mobo, receiver and dip switchTheres the bugger. The logitech receiver is there, under the black heat shrink.2016-07-23T03:29:41+00:00monthlyhttps://aylo6061.com/2011/11/10/how-i-feel-when-people-visit-the-download-page/https://aylo6061.com/wp-content/uploads/2011/11/askfor.jpgaskforThis is how I feel.2016-07-23T03:29:36+00:00monthlyhttps://aylo6061.com/2011/11/15/debugging-a-preamp/https://aylo6061.com/wp-content/uploads/2011/11/fx_1.jpgfx_1Fx-1 mic that was attached to the amphttps://aylo6061.com/wp-content/uploads/2011/11/2241.jpg9v battery clipOne of these buggers2016-07-23T03:29:32+00:00monthlyhttps://aylo6061.com/2011/12/09/live-streaming-webcam/https://aylo6061.com/wp-content/uploads/2011/12/works.jpgworks!Streaming Live!https://aylo6061.com/wp-content/uploads/2011/12/issstarter.jpgissstarterIIS interfacehttps://aylo6061.com/wp-content/uploads/2011/12/msexpression.jpgmsexpressionMS Expressionhttps://aylo6061.com/wp-content/uploads/2011/12/iis2.jpgiis2Click this one! Then OKhttps://aylo6061.com/wp-content/uploads/2011/12/iis1.jpgHow to turn on Windows featuresTurn on/off Windows features with this button2016-07-23T03:29:27+00:00monthlyhttps://aylo6061.com/2011/12/13/downloads-fixed-empty-blog-post-fixed/https://aylo6061.com/wp-content/uploads/2011/12/screenshot.pngScreenshotAm I trapped in The Matrix?https://aylo6061.com/wp-content/uploads/2011/12/1.png1Oh no!2016-07-23T03:29:19+00:00monthlyhttps://aylo6061.com/2011/12/14/transformation-2-0-risd/https://aylo6061.com/wp-content/uploads/2011/12/imag0327.jpgIMAG0327The ring of BAD. Note growth around the edges of the plate.https://aylo6061.com/wp-content/uploads/2011/12/imag0343.jpgIMAG0343Transformants expressing GFP, which is fluorescing under a blue LEDhttps://aylo6061.com/wp-content/uploads/2011/12/imag0337.jpgIMAG0337Transformants?2016-07-23T03:29:13+00:00monthlyhttps://aylo6061.com/2011/12/18/diy-bio-postcards-bioluminescent-dinoflagellates-oh-my/https://aylo6061.com/wp-content/uploads/2011/12/img_0001.jpgIMG_0001The little speck in the middle is P. lunula2016-07-23T03:29:06+00:00monthlyhttps://aylo6061.com/2012/01/17/paint-can-forge/https://aylo6061.com/wp-content/uploads/2012/01/p1010688.jpgP1010688The porta-pit of Hell.https://aylo6061.com/wp-content/uploads/2012/01/p1010682.jpgP10106822016-07-23T03:28:58+00:00monthlyhttps://aylo6061.com/2011/12/26/laser-projector/2016-07-23T03:28:48+00:00monthlyhttps://aylo6061.com/2012/01/09/adventure-kelsey-mine-part-i-in-which-we-execute-a-near-fruitless-search/https://aylo6061.com/wp-content/uploads/2012/01/derp.jpgoopsAs you can see, we are not even close to "kind of where we are supposed to be"2016-07-23T03:28:44+00:00monthlyhttps://aylo6061.com/2012/01/13/adventure-kelsey-mine-part-ii-in-which-we-actually-find-the-kelsey/https://aylo6061.com/wp-content/uploads/2012/01/p1030692.jpgP1030692Sign posted outside of the enterance. Ought to say "MINERS MUST BE THIS TALL TO RIDE THE ORE CART". Watch your head!https://aylo6061.com/wp-content/uploads/2012/01/p1030691.jpgP1030691The enterance to the Kelsey mine. Miners must be this tall to enter.https://aylo6061.com/wp-content/uploads/2012/01/p1030831.jpgP1030831The view from the top of the second falls. The steep, narrow path on the left of the photo appears to be cut out of the rock, and was our ascent route.https://aylo6061.com/wp-content/uploads/2012/01/p1030796.jpgP1030796https://aylo6061.com/wp-content/uploads/2012/01/p1030793.jpgP1030793Sunlight never looked so good.https://aylo6061.com/wp-content/uploads/2012/01/p1030787.jpgP1030787Coppery things!https://aylo6061.com/wp-content/uploads/2012/01/p1030762.jpgP1030762https://aylo6061.com/wp-content/uploads/2012/01/p1030745.jpgP1030745The flooded passage. Difficult to photograph, but very beautiful.https://aylo6061.com/wp-content/uploads/2012/01/p1030728.jpgP1030728https://aylo6061.com/wp-content/uploads/2012/01/p1030712.jpgP10307122020-10-30T01:51:13+00:00monthlyhttps://aylo6061.com/2012/01/18/paint-can-forge-day-two-overmelted/https://aylo6061.com/wp-content/uploads/2012/01/p1010734.jpgP1010734A super hot jet of gas is coming out of the charcoal. The forge has a loud roar at this point, and sparks and exploding charcoal come our regularlyhttps://aylo6061.com/wp-content/uploads/2012/01/p1010713.jpgP1010713The porta-pit in action!https://aylo6061.com/wp-content/uploads/2012/01/p1010696.jpgP1010696Knife, sitting pretty and riding around on the magnet stickhttps://aylo6061.com/wp-content/uploads/2012/01/p1010743.jpgP1010743Ruined blades-to-behttps://aylo6061.com/wp-content/uploads/2012/01/p1010691.jpgP1010691https://aylo6061.com/wp-content/uploads/2012/01/p1010690.jpgP1010690https://aylo6061.com/wp-content/uploads/2012/01/p1010739.jpgP1010739TOO HOT! But the whole thing finally did get past the critical point2016-07-23T03:28:24+00:00monthlyhttps://aylo6061.com/2012/01/20/paint-can-forge-day-three-hell-and-back/https://aylo6061.com/wp-content/uploads/2012/01/p1010897-e1327050650701.jpgP1010897My brother decided to go all B&W during the heat-treat.https://aylo6061.com/wp-content/uploads/2012/01/p1010811.jpgP1010811https://aylo6061.com/wp-content/uploads/2012/01/p1010762.jpgP1010762https://aylo6061.com/wp-content/uploads/2012/01/p1010764.jpgP1010764https://aylo6061.com/wp-content/uploads/2012/01/p1010822.jpgP1010822That copper pipe is orange hot. Steel was a similar color, indicating the forge was at about 930 F or 1705 C2016-07-23T03:28:19+00:00monthlyhttps://aylo6061.com/2012/01/21/how-to-build-a-paint-can-forge/https://aylo6061.com/wp-content/uploads/2012/01/p1010905.jpgP1010905https://aylo6061.com/wp-content/uploads/2012/01/p1010933.jpgP1010933Diagram of the Forge 2.0https://aylo6061.com/wp-content/uploads/2012/01/p1010838.jpgP1010838Keepy your eyebrows safe, yo.https://aylo6061.com/wp-content/uploads/2012/01/p1010810.jpgP1010810Orange HOT!https://aylo6061.com/wp-content/uploads/2012/01/p1010802.jpgP1010802https://aylo6061.com/wp-content/uploads/2012/01/p1010841.jpgP1010841Watch out for sparks! And exploders!https://aylo6061.com/wp-content/uploads/2012/01/p1010804.jpgP1010804Just back from the dirt minehttps://aylo6061.com/wp-content/uploads/2012/01/p1010776.jpgP1010776The inside, showing the notched copper pipehttps://aylo6061.com/wp-content/uploads/2012/01/p1010774.jpgP1010774Forge 2.0 almost all put togetherhttps://aylo6061.com/wp-content/uploads/2012/01/p1010773.jpgP1010773Cut out the black parts to make a face. Or a forge, you decide!2016-07-23T03:28:14+00:00monthlyhttps://aylo6061.com/2012/01/22/making-a-knife-from-a-file-the-blade/https://aylo6061.com/wp-content/uploads/2012/01/p1010938.jpgP1010938It is starting to look like the knife I designed!https://aylo6061.com/wp-content/uploads/2012/01/p10109161.jpgP1010916Note light straw color.https://aylo6061.com/wp-content/uploads/2012/01/p1010896.jpgP1010896https://aylo6061.com/wp-content/uploads/2012/01/p10106901.jpgP1010690Setup for drilling holes in the overmelted knife.https://aylo6061.com/wp-content/uploads/2012/01/p1010796.jpgP1010796https://aylo6061.com/wp-content/uploads/2012/01/p1010782.jpgP1010782Ink testhttps://aylo6061.com/wp-content/uploads/2012/01/p1010787.jpgP1010787The knife after some rough grindinghttps://aylo6061.com/wp-content/uploads/2012/01/p1010770.jpgP1010770Straight air-coolin'https://aylo6061.com/wp-content/uploads/2012/01/p1010937.jpgP1010937https://aylo6061.com/wp-content/uploads/2012/01/p1010935.jpgP1010935different blades for different applications!2016-07-23T03:28:01+00:00monthlyhttps://aylo6061.com/2012/02/01/secret-knowledge-revobots/2016-07-23T03:27:54+00:00monthlyhttps://aylo6061.com/2012/02/02/5-gallon-saltwater-tank-build/https://aylo6061.com/wp-content/uploads/2012/02/188264_10150248113676840_699561839_7910019_3555725_n.jpg188264_10150248113676840_699561839_7910019_3555725_nRIP Magnum, the best fishhttps://aylo6061.com/wp-content/uploads/2012/01/img_0027.jpgIMG_0027Magical live rockhttps://aylo6061.com/wp-content/uploads/2012/01/img_0028-e1328208947623.jpgIMG_0028The rock in the tank!https://aylo6061.com/wp-content/uploads/2012/01/img_0020.jpgIMG_0020Featherduster polyp?https://aylo6061.com/wp-content/uploads/2012/01/img_0019.jpgIMG_00192016-07-23T03:27:49+00:00monthlyhttps://aylo6061.com/2012/02/03/bioethics-dont-ship-invasive-species/2016-07-23T03:27:41+00:00monthlyhttps://aylo6061.com/2012/02/06/adventure-dawn-mine-at-dusk/https://aylo6061.com/wp-content/uploads/2012/02/p1010609.jpgP1010609Los Angeles at night.https://aylo6061.com/wp-content/uploads/2012/02/p1010600.jpgP1010600Chloe Points out some especially trecherous parts of the trailhttps://aylo6061.com/wp-content/uploads/2012/02/p1010598.jpgP1010598Watch out for drops!https://aylo6061.com/wp-content/uploads/2012/02/p1010595.jpgP1010595Suddenly, it was very darkhttps://aylo6061.com/wp-content/uploads/2012/02/p1010584.jpgP1010584View of forested area from the mine entrancehttps://aylo6061.com/wp-content/uploads/2012/02/p1010580.jpgP1010580Socks! Socks! Socks!https://aylo6061.com/wp-content/uploads/2012/02/p1010575-e1328516365390.jpgP1010575Chloe managing to not get wet.https://aylo6061.com/wp-content/uploads/2012/02/p1010558-e1328515958719.jpgP1010558You can't tell, but there is an underground waterfall behind us.https://aylo6061.com/wp-content/uploads/2012/02/p1010551-e1328515857739.jpgP1010551The source of the waterhttps://aylo6061.com/wp-content/uploads/2012/02/p1010548.jpgP1010548The way is not shut, but it is certainly wet!2016-07-23T03:27:36+00:00monthlyhttps://aylo6061.com/2012/02/09/transformation-3-0-today-i-learned-about-ampicillin/2016-07-23T03:27:17+00:00monthlyhttps://aylo6061.com/2012/02/16/toool-boston-meeting-pick-locks-get-loot/https://aylo6061.com/wp-content/uploads/2012/02/2012-02-16_02-03-34_845.jpg2012-02-16_02-03-34_845Swag from TOOOL Boston meeting2016-07-23T03:26:51+00:00monthlyhttps://aylo6061.com/2012/02/23/lockpicking-saves-the-day-or-foozeball-table/https://aylo6061.com/wp-content/uploads/2012/02/2012-02-18_19-27-50_217.jpg2012-02-18_19-27-50_217Picked!https://aylo6061.com/wp-content/uploads/2012/02/2012-02-18_19-26-25_960.jpg2012-02-18_19-26-25_960The chutes in question2016-07-23T03:26:46+00:00monthlyhttps://aylo6061.com/2012/02/24/blyes-bioluminescent-yeast-estrogen-assay/https://aylo6061.com/wp-content/uploads/2012/02/blyes.pngBLYESBLYES: hER-alpha receptor is encoded by chromosomal DNA. Activation of the receptor turns on production of luxA and luxB under the control of bidirectional EREs. This enzyme reacts with the FRP produced by the constitutively on pUTK404 plasmid. pUTK404 also encodes LuxCDE, which allows the recycling of FRP. frp, and Lux genes CDE have IRES (internal ribosome entry sites) in-between them to ensure proper transcriptionhttps://aylo6061.com/wp-content/uploads/2012/02/bactolux.pngbactoluxthe Lux operon. LuxA and LuxB code for the luciferase enzyme, while LuxCDE code for the substrate and complex that recycle ithttps://aylo6061.com/wp-content/uploads/2012/02/yes.pngYES assay systemYES assay system. Circles are rough sketches of the plasmid. Estrogen interacts with receptor coded by pSCW231-hER, which causes the translation and transcription of b-galactosidasehttps://aylo6061.com/wp-content/uploads/2012/02/diagram1.pngestrogen flow chartFlow of estrogenic compounds2016-07-23T03:26:42+00:00monthlyhttps://aylo6061.com/2012/02/26/revobots-week-0-a-success-overall/https://aylo6061.com/wp-content/uploads/2012/02/igp6403.jpg_IGP6403Oh Windows 7, y u no work?https://aylo6061.com/wp-content/uploads/2012/02/igp6410.jpg_IGP6410https://aylo6061.com/wp-content/uploads/2012/02/igp6401.jpg_IGP6401Now I know how the guy in the blue feels. He is one of our professors!https://aylo6061.com/wp-content/uploads/2012/02/igp6399.jpgAvery Says: Microcontrollers, yoMicrocontrollers, Yo.https://aylo6061.com/wp-content/uploads/2012/02/igp6389.jpg_IGP6389https://aylo6061.com/wp-content/uploads/2012/02/igp6386.jpg_IGP6386Lots of people=lots of prep2016-07-23T03:26:36+00:00monthlyhttps://aylo6061.com/2012/02/27/the-gfp-project/https://aylo6061.com/wp-content/uploads/2012/02/p1010470.jpgP1010470https://aylo6061.com/wp-content/uploads/2012/02/p1010469.jpgsomething is cooking at BOSSLABSomething is cooking at BOSSLAB!2016-07-23T03:26:32+00:00monthlyhttps://aylo6061.com/2012/02/28/diybio-streaking-plates-to-isolate-colonies/https://aylo6061.com/wp-content/uploads/2012/02/2011-04-25_01-32-06_617.jpg2011-04-25_01-32-06_617Isolated colonies. Also note initial thick streaks in to the top of the plate, and then the less continuous sreaks in the bottom right, and the thinnest concentration of isolated colonies on the bottom lefthttps://aylo6061.com/wp-content/uploads/2012/02/2011-04-25_01-31-22_591.jpg2011-04-25_01-31-22_591Clear streaking pattern, but there are too many bacteria, and it looks like I overlapped my first zig-zag with my last zig-zag! oops.https://aylo6061.com/wp-content/uploads/2012/02/img_0781.jpgIMG_0781Good, isolated colonies2016-07-23T03:26:08+00:00monthlyhttps://aylo6061.com/2012/02/29/revobots-week-2-gotta-switch-up-the-teaching-plan/https://aylo6061.com/wp-content/uploads/2012/02/igp6442.jpg_IGP6442https://aylo6061.com/wp-content/uploads/2012/02/igp6438.jpg_IGP6438This week we talked about sensors. The amount of light on the LDR changes the brightness of the LED2016-07-23T03:26:03+00:00monthlyhttps://aylo6061.com/2012/03/02/diybio-stickers-ordered/https://aylo6061.com/wp-content/uploads/2012/03/selection_010.jpegSelection_010Bam. Sharp lookin' sticker!2016-07-23T03:26:00+00:00monthlyhttps://aylo6061.com/2012/03/04/revobots-week-3-halfway-there/https://aylo6061.com/wp-content/uploads/2012/03/igp6518.jpg_IGP6518Inspiring student gets breakbeam sensor to work.https://aylo6061.com/wp-content/uploads/2012/03/igp6513.jpg_IGP6513Debug ALL the problems!https://aylo6061.com/wp-content/uploads/2012/03/igp6496.jpg_IGP6496In this class, the challenge was to build a breakbeam sensor.https://aylo6061.com/wp-content/uploads/2012/03/igp6488.jpg_IGP6488Another good turnout. Obviously, this is not a picture of everyone who attended...2016-07-23T03:25:34+00:00monthlyhttps://aylo6061.com/2012/03/05/the-gfp-project-week-1-hands-on-transformation/https://aylo6061.com/wp-content/uploads/2012/03/p1010604.jpgP1010604Spread the bacteria around with the sterile loophttps://aylo6061.com/wp-content/uploads/2012/03/p1010581.jpgP1010581https://aylo6061.com/wp-content/uploads/2012/03/p1010591.jpgP1010591Heat shock!https://aylo6061.com/wp-content/uploads/2012/03/p1010579.jpgP1010579Adding tiny amounts of DNA means tiny pipette tips!https://aylo6061.com/wp-content/uploads/2012/03/p1010519.jpgP1010519Gather all the things! Everything didn't fit in this picture, but this is a lot of stuff!https://aylo6061.com/wp-content/uploads/2012/03/titlepic.jpgtitlepicThe shirt says it all.https://aylo6061.com/wp-content/uploads/2012/03/p1010570.jpgP1010570https://aylo6061.com/wp-content/uploads/2012/03/p1010569.jpgP1010569https://aylo6061.com/wp-content/uploads/2012/03/p1010557.jpgP1010557https://aylo6061.com/wp-content/uploads/2012/03/p1010555.jpgP10105552016-07-23T03:25:25+00:00monthlyhttps://aylo6061.com/2012/03/07/the-gfp-project-week-1-a-lesson-in-patience-and-ingenuity/https://aylo6061.com/wp-content/uploads/2012/03/fermbas_4.jpgfermbas_4cell growth phase chart, found via the googlehttps://aylo6061.com/wp-content/uploads/2012/03/igp5196.jpg_IGP5196BAM. GFP!https://aylo6061.com/wp-content/uploads/2012/03/igp5193.jpg_IGP5193Arduino: the alternative lighting platform for molecular biologyhttps://aylo6061.com/wp-content/uploads/2012/03/igp5186.jpg_IGP5186Perfect little colonies!2016-07-23T03:25:20+00:00monthlyhttps://aylo6061.com/2012/03/10/revobots-week-4-rebobot-factory/https://aylo6061.com/wp-content/uploads/2012/03/igp6686.jpg_IGP6686Materialshttps://aylo6061.com/wp-content/uploads/2012/03/igp6683.jpg_IGP6683https://aylo6061.com/wp-content/uploads/2012/03/igp6673.jpg_IGP6673Robot Factory!https://aylo6061.com/wp-content/uploads/2012/03/igp6671.jpg_IGP6671Attendance was not too shabbyhttps://aylo6061.com/wp-content/uploads/2012/03/igp6664.jpg_IGP6664https://aylo6061.com/wp-content/uploads/2012/03/igp6662.jpg_IGP66622016-07-23T03:25:15+00:00monthlyhttps://aylo6061.com/2012/03/12/gfp-project-week-2-extractifying-gfp/https://aylo6061.com/wp-content/uploads/2012/03/igp5276.jpg_IGP5276GFP! GFP has an excitation peak in the blue light spectrum. Unfortunately, the blue LED is comparatively bright. Gotta find a green pass filter!https://aylo6061.com/wp-content/uploads/2012/03/p1010762.jpgP1010762https://aylo6061.com/wp-content/uploads/2012/03/p1010761.jpgP1010761https://aylo6061.com/wp-content/uploads/2012/03/p1010755.jpgP1010755https://aylo6061.com/wp-content/uploads/2012/03/p1010752.jpgP1010752https://aylo6061.com/wp-content/uploads/2012/03/p1010750.jpgP1010750https://aylo6061.com/wp-content/uploads/2012/03/p1010749.jpgnon-fluorescent GFPThe resin glows now, but the supernatant does not!https://aylo6061.com/wp-content/uploads/2012/03/p1010739.jpgGFP in binding buffer sitting on the HIC resinGFP in binding buffer sitting on the HIC resinhttps://aylo6061.com/wp-content/uploads/2012/03/p1010734.jpgGFP after high salt binding buffer After the binding buffer has been addedhttps://aylo6061.com/wp-content/uploads/2012/03/p1010729.jpgsupernatant lysate in a clean tubesupernatant lysate in a clean tube2016-07-23T03:25:11+00:00monthlyhttps://aylo6061.com/2012/03/12/diybio-stickers-have-arrived/https://aylo6061.com/wp-content/uploads/2012/03/igp5294.jpgGFP DIYBIO STICKER!Aww yea.https://aylo6061.com/wp-content/uploads/2012/03/igp5272.jpgDIYBIO stickerSwag!2016-07-23T03:24:54+00:00monthlyhttps://aylo6061.com/2012/03/12/revobots-manny-the-manual-line-following-robot/https://aylo6061.com/wp-content/uploads/2012/03/igp5257.jpg_IGP5257Oh no! Hard to port!https://aylo6061.com/wp-content/uploads/2012/03/igp5256.jpg_IGP5256https://aylo6061.com/wp-content/uploads/2012/03/igp5246.jpg_IGP5246https://aylo6061.com/wp-content/uploads/2012/03/igp5245.jpg_IGP5245Manny, the manually operated robot2016-07-23T03:24:47+00:00monthlyhttps://aylo6061.com/2012/03/27/the-gfp-project-one-step-backward-two-steps-forward/2016-07-23T03:24:43+00:00monthlyhttps://aylo6061.com/2012/04/03/gfp-project-week-three-dna-3/https://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_19-07-13_627.jpg2012-04-01_19-07-13_627Purified plasmid DNAhttps://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_18-48-00_839.jpg2012-04-01_18-48-00_839Eloution!https://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_18-34-36_728.jpg2012-04-01_18-34-36_728Spin Columnhttps://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_18-07-01_778.jpg2012-04-01_18-07-01_778Pelleted Cell Debrishttps://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_17-50-27_459.jpg2012-04-01_17-50-27_459https://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_17-49-53_78.jpg2012-04-01_17-49-53_78https://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_17-49-46_932.jpg2012-04-01_17-49-46_932Halted Lysishttps://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_17-47-44_132.jpg2012-04-01_17-47-44_132https://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_17-46-54_758.jpg2012-04-01_17-46-54_758Lysed Cellshttps://aylo6061.com/wp-content/uploads/2012/04/2012-04-01_17-46-50_805.jpg2012-04-01_17-46-50_805Resuspended Cells2016-07-23T03:24:34+00:00monthlyhttps://aylo6061.com/2012/04/13/gfp-project-dna-quantification/https://aylo6061.com/wp-content/uploads/2012/04/p4110585.jpgNanodrop machineThis is the nanodrop, a machine for quantifying DNA.https://aylo6061.com/wp-content/uploads/2012/04/p4110582.jpgDNA quantificationThe interface for the nanodrop. The prep from BOSSLAB is at the top of the queue!2016-07-23T03:24:27+00:00monthlyhttps://aylo6061.com/2012/04/14/deizens-of-the-saltwater-tank-3/https://aylo6061.com/wp-content/uploads/2012/04/p4080271.jpgNassarius snailThis is the nassarius snail, in the middle. it looks like he is hitching a ride on a Florida cerithhttps://aylo6061.com/wp-content/uploads/2012/04/p4090413.jpgflorida cerithThis big guy is a florida cerith!https://aylo6061.com/wp-content/uploads/2012/04/p4080281.jpgNerite SnailHeres a nerite snailhttps://aylo6061.com/wp-content/uploads/2012/04/p4080265.jpgfetherdusterThis is a featherdusterhttps://aylo6061.com/wp-content/uploads/2012/04/p4080242.jpgdwarf cerith snailsA multitude of dwarf cerith snailshttps://aylo6061.com/wp-content/uploads/2012/04/p4080238.jpgcyanobacteria!Cyanobacteria!2016-07-23T03:24:20+00:00monthlyhttps://aylo6061.com/2012/04/15/baby-sea-animals-everywhere/https://aylo6061.com/wp-content/uploads/2012/04/p4080254.jpgcopeopodLast but not least, a copeopodhttps://aylo6061.com/wp-content/uploads/2012/04/p4110618.jpgBaby snailBaby snail!https://aylo6061.com/wp-content/uploads/2012/04/p4090418.jpgJust a blurry view of my aquarium...or is it?Just a blurry view of my aquarium...or is it?https://aylo6061.com/wp-content/uploads/2012/04/p4090411_01.jpgbaby tube worm/featherdusterUpon closer inspection, there is a baby tube worm/featherduster!https://aylo6061.com/wp-content/uploads/2012/04/p4090411.jpgJust a blurry view of my aquarium...or is it?Just a blurry view of my aquarium...or is it?https://aylo6061.com/wp-content/uploads/2012/04/p4090397.jpgBifurcated bristlewormThis appears to be a bifurcated bristlewormhttps://aylo6061.com/wp-content/uploads/2012/04/p4090371.jpgI suspect this is a bristlewormI suspect this is a bristleworm2016-07-23T03:24:15+00:00monthlyhttps://aylo6061.com/2012/04/20/mini-maker-faire-cambridge-science-festival/https://aylo6061.com/wp-content/uploads/2012/04/p4200072.jpgDIY@mini maker fairReally bad photo. I apologize...2016-07-23T03:24:08+00:00monthlyhttps://aylo6061.com/2012/04/23/revobots-56-the-end-of-the-revobots-story/https://aylo6061.com/wp-content/uploads/2012/04/p42302521.jpgREVOBotTop view of the 'bothttps://aylo6061.com/wp-content/uploads/2012/04/p4140022.jpgRevobots!Students in REVOBotshttps://aylo6061.com/wp-content/uploads/2012/04/p4140018.jpgA student testing a 'bot in the darkA student testing a 'bot in the dark2016-07-23T03:24:03+00:00monthlyhttps://aylo6061.com/2012/04/25/genomes-environments-and-traits-confrence/https://aylo6061.com/wp-content/uploads/2012/04/p4250262.jpgGETS conferenceJason Bobe moderates a discussion with George Church and Geraldine Hamilton about personalized medicine microfluidic devices2016-07-23T03:23:50+00:00monthlyhttps://aylo6061.com/2012/05/01/gene-cloning-successful/https://aylo6061.com/wp-content/uploads/2012/05/aga23.jpgaga23https://aylo6061.com/wp-content/uploads/2012/05/aga45.jpgaga452016-07-23T03:23:46+00:00monthlyhttps://aylo6061.com/2012/05/11/inspiring-people-cellfyre-andreas-sturm-and-chris-templeman/2016-07-23T03:23:40+00:00monthlyhttps://aylo6061.com/2012/05/25/how-do-i-get-started-in-diybio/2016-07-23T03:23:33+00:00monthlyhttps://aylo6061.com/2012/06/05/malaguti-pilot-a-new-project/https://aylo6061.com/wp-content/uploads/2012/06/p6020925.jpgpetcock with no fuel lineSomething is missing here...https://aylo6061.com/wp-content/uploads/2012/06/p6020927.jpgfuel line routed around stash boxfuel line and filter routed around stash boxhttps://aylo6061.com/wp-content/uploads/2012/06/p6020924.jpgM02 MO2 motor motori morini no sparkplugSomething is missing here too! What could it be...?https://aylo6061.com/wp-content/uploads/2012/06/p6020923.jpgMalagut PilotThe " 'guti ", a malaguti pilot from the 70's2016-07-23T03:23:28+00:00monthlyhttps://aylo6061.com/2012/06/11/malaguti-update-671299/https://aylo6061.com/wp-content/uploads/2012/06/p6090929.jpgmalaguti pilot electrical snafuA picture showing the electrical SNAFUhttps://aylo6061.com/wp-content/uploads/2012/06/p6090932.jpgMissing CEV tail light on malaguti pilotWhats missing? The tail light is missing...https://aylo6061.com/wp-content/uploads/2012/06/p6090931.jpgMalaguti pilotLooking good despite being about 40 years old.2016-07-23T03:23:23+00:00monthlyhttps://aylo6061.com/2012/06/11/visited-the-treatland-tv-in-real-life/https://aylo6061.com/wp-content/uploads/2012/06/treats.jpgTreats!Treats is here!https://aylo6061.com/wp-content/uploads/2012/06/p6110948.jpgTreat street signTreat Streethttps://aylo6061.com/wp-content/uploads/2012/06/p6110945.jpgNot treatland. SRS BZNSS HONDASNot treatland. SRS BZNSS HONDAShttps://aylo6061.com/wp-content/uploads/2012/06/p6110944.jpgis this treatland?is this treatland?2016-07-23T03:23:16+00:00monthlyhttps://aylo6061.com/2012/06/14/fbi-diybio-outreach-workshop/2016-07-23T03:23:11+00:00monthlyhttps://aylo6061.com/2012/06/24/diybio-fbi-outreach-conference-san-fransisco/https://aylo6061.com/wp-content/uploads/2012/06/p6130962.jpgThomas take a picture of the bathroomI took a picture of Tomas taking a picture of the bathroom at Genspace.https://aylo6061.com/wp-content/uploads/2012/06/p6140971.jpgGenomikon sequential DNA ligation kitThen we played with the Genomikon kit!https://aylo6061.com/wp-content/uploads/2012/06/p6140980.jpgInternational DIYBIOcountries from left to right: USA, The Netherlands (behind the benches), Finland, Denmark, Germany, Turkeyhttps://aylo6061.com/wp-content/uploads/2012/06/p6130953.jpgDIYBIO FBI conferenceThe room was pretty full! I didn't know there were this many diybiologists.https://aylo6061.com/wp-content/uploads/2012/06/p6130955.jpgFBI DIYBIO conference posterActually, we were in Walnut Creek, but it is close enough to call it San Fransisco!2016-07-23T03:23:07+00:00monthlyhttps://aylo6061.com/2012/07/03/morini-mo2-clutch-cable-replacement/https://aylo6061.com/wp-content/uploads/2012/07/p6230987.jpgthe right kind of clutch cable for an m02 morini engineThe right kind of clutch cable for an m02 morinihttps://aylo6061.com/wp-content/uploads/2012/07/p6230990.jpgThe wrong kind of clutch cableThe wrong kind of nubbin!2016-07-23T03:22:53+00:00monthlyhttps://aylo6061.com/2012/07/03/mo-moped-repairs/https://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_22-29-21_754.jpgsuprisingly good led lampSurprisingly high quality LED lamp and improvised lock-hammerhttps://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_21-58-54_984.jpgfixing mopedFixing my moped!https://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_21-00-46_631.jpgbearings clutchClutch case and some bearings that came out with it! If you look hard you can find the nubbin.https://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_20-59-20_101.jpgmornini m02 clutch and transThe clutch of the morini m02https://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_20-57-36_821.jpgMoped stuffAnother night of moped work. Goo everywherehttps://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_20-57-30_226.jpgBRIGHT LEDS!Bright LED lights. Don't look at these; they will blind you!https://aylo6061.com/wp-content/uploads/2012/07/2012-06-30_19-35-46_82.jpgmotor oil everywhereOil everywhere! Next time I will drain the oil out with the bolts that are specially made for draining the oil out...https://aylo6061.com/wp-content/uploads/2012/07/p6230986.jpgp62309862016-07-23T03:22:47+00:00monthlyhttps://aylo6061.com/2012/07/06/got-some-good-stuff-from-idt-today/https://aylo6061.com/wp-content/uploads/2012/07/p7051029.jpgBluegene primersBluegene primers!https://aylo6061.com/wp-content/uploads/2012/07/p7051028.jpgIDT primer envelopeWHATS IN THE ENVELOOOOPPEEE?2016-07-23T03:22:42+00:00monthlyhttps://aylo6061.com/2012/07/31/gelgreen-as-good-as-advertised/https://aylo6061.com/wp-content/uploads/2012/07/p7291096.jpgGelGreen with Blue LEDSCheck out the bands on the right! Its a DNA ladder. Its pretty smiley because I was running at high amperage just to test the new stain.2016-07-23T03:22:36+00:00monthlyhttps://aylo6061.com/2012/07/31/more-morini-repairs/https://aylo6061.com/wp-content/uploads/2012/07/2012-07-21_16-41-49_724.jpgfiling the headThank goodness for tools. I had to take about .5 cm off the whole outside of the exhaust header.https://aylo6061.com/wp-content/uploads/2012/07/2012-07-21_16-42-22_15.jpgpipe comparisonOld pipe has a nice bend so i have more clearance, new pipe is not full of carbon. Maybe the old one will make a comeback someday.https://aylo6061.com/wp-content/uploads/2012/07/2012-07-21_16-43-28_613.jpgGasket doesent fit head of old exhaustAs you can see, the new gasket doesn't fit the old exhaust header. Or the new header, for that matter. The holes are too far apart.https://aylo6061.com/wp-content/uploads/2012/07/2012-07-21_16-48-36_983.jpgmounting bracket for mk3 exhaustThe mounting bracket needed to be enlarged. Lots of filing was required to do this.2016-07-23T03:22:29+00:00monthlyhttps://aylo6061.com/2012/07/31/woodruff-key-removal-and-replacement/https://aylo6061.com/wp-content/uploads/2012/07/p7301123.jpgmagneto assyHappily installed in the flywheel.https://aylo6061.com/wp-content/uploads/2012/07/p7301118.jpgold key new keyold key, new key!https://aylo6061.com/wp-content/uploads/2012/07/p7301108.jpgwoodruff keyAfter some judicious smacking, the key came loosehttps://aylo6061.com/wp-content/uploads/2012/07/p7301104.jpgsheared woodruff keyIf you look closely, you can see the key that is totally sheared off in the shaft.2016-07-23T03:22:21+00:00monthlyhttps://aylo6061.com/2012/07/31/morini-ignition-magneto-rebuild/https://aylo6061.com/wp-content/uploads/2012/07/p7301126.jpgCondenser on the bikeHere it is on the bike. The red wire goes to the ignition.https://aylo6061.com/wp-content/uploads/2012/07/p7291094.jpgstator wired upHere it is, all soldered up.https://aylo6061.com/wp-content/uploads/2012/07/p7291090.jpgstator and condenserThe condenser should slide right into the stator plate. Mine was an 18 mm wide X 25 mm tall condenserhttps://aylo6061.com/wp-content/uploads/2012/07/p7291084.jpgstator plateGet all your parts together on your workbench/cardboard box. Don't forget a random bag of chips!2016-07-23T03:22:14+00:00monthlyhttps://aylo6061.com/2012/08/08/the-morini-roars/https://aylo6061.com/wp-content/uploads/2012/08/p8041220.jpgb6hs vs b6esB6HS vs B6ES- the ES is the longer one.https://aylo6061.com/wp-content/uploads/2012/08/p8041215.jpgOil fouling?Oil fouling?https://aylo6061.com/wp-content/uploads/2012/08/p8051221.jpgcruise controlGhetto Cruise Control (caps lock for my throttle)https://aylo6061.com/wp-content/uploads/2012/08/p8051222.jpgmo2 top endAnother day working on the 'ped2016-07-23T03:22:04+00:00monthlyhttps://aylo6061.com/2012/08/17/upper-dawn-mine/https://aylo6061.com/wp-content/uploads/2012/08/p1020206.jpgIts a long way downIts a long way down. That branch in the foreground is about 3 in across, while the I beams in the middle are about 8-6 in across.https://aylo6061.com/wp-content/uploads/2012/08/p1020201.jpgdoin the boltDoin' the Bolt at the entrance to the Upper Dawnhttps://aylo6061.com/wp-content/uploads/2012/08/p1020199.jpgpeace out upper dawnLeaving the upper entrance is just as difficult as entering.https://aylo6061.com/wp-content/uploads/2012/08/p1020189.jpgcreepy tunnerlCreepy tunnel!https://aylo6061.com/wp-content/uploads/2012/08/p1020195.jpgpit of doomPit of doom/deep shafthttps://aylo6061.com/wp-content/uploads/2012/08/p1020197.jpgexiting the upper dawnThe Upper entrance, looking outhttps://aylo6061.com/wp-content/uploads/2012/08/p1020193.jpgP1020193https://aylo6061.com/wp-content/uploads/2012/08/p1020185.jpglooking down from the underground waterfalllooking down from the underground waterfallhttps://aylo6061.com/wp-content/uploads/2012/08/p1020186.jpglooking down from the underground waterfall2looking down from the underground waterfallhttps://aylo6061.com/wp-content/uploads/2012/08/p1020181.jpglizard in the lower dawnlarge lizard in the lower dawn2016-07-23T03:19:42+00:00monthlyhttps://aylo6061.com/2012/09/29/atmega32u4-breakout/https://aylo6061.com/wp-content/uploads/2012/09/top.pngtophttps://aylo6061.com/wp-content/uploads/2012/09/bottom.pngbottom2016-07-23T03:19:35+00:00monthlyhttps://aylo6061.com/2013/07/01/boston-hardware-startup-meetup-4/2016-07-23T03:19:28+00:00monthlyhttps://aylo6061.com/2013/07/11/indoxyl-an-incarnation-of-the-usbtiny-isp/https://aylo6061.com/wp-content/uploads/2013/07/20130615_011145.jpg20130615_011145New Indoxyl Programmerhttps://aylo6061.com/wp-content/uploads/2012/02/igp5159.jpgAttiny usbisp Programmer next to LEDProgrammer next to LED2016-07-23T03:19:18+00:00monthlyhttps://aylo6061.com/2013/07/19/gkos-keyboard-adventures/2016-07-23T03:19:10+00:00monthlyhttps://aylo6061.com/2013/07/20/nice-pictures-of-gel-electrophoresis/https://aylo6061.com/wp-content/uploads/2013/07/compare.pngcompareWinner is the XS 1000, as you can see the difference in the dark colors!https://aylo6061.com/wp-content/uploads/2013/07/img_4600.jpgIMG_4600https://aylo6061.com/wp-content/uploads/2013/07/img_4599.jpgIMG_4599https://aylo6061.com/wp-content/uploads/2013/07/img_4594.jpgIMG_4594Woah man, it's like instagramhttps://aylo6061.com/wp-content/uploads/2013/07/img_4589.jpgIMG_4589Gel Photography Devicehttps://aylo6061.com/wp-content/uploads/2013/07/20130715_193243.jpg20130715_1932432016-07-23T03:18:37+00:00monthlyhttps://aylo6061.com/2013/07/22/diy-vacuum-manifold/https://aylo6061.com/wp-content/uploads/2013/07/vac.jpgvachttps://aylo6061.com/wp-content/uploads/2013/07/manifold1.jpgmanifoldhttps://aylo6061.com/wp-content/uploads/2013/07/delrin_fold1.pngdelrin_fold2016-07-23T03:18:30+00:00monthlyhttps://aylo6061.com/2013/07/22/chorded-keyboard-f1rst-pr1nt/https://aylo6061.com/wp-content/uploads/2013/07/img_4571.jpgIMG_4571https://aylo6061.com/wp-content/uploads/2013/07/img_4569.jpgIMG_4569https://aylo6061.com/wp-content/uploads/2013/07/img_4567.jpgIMG_4567https://aylo6061.com/wp-content/uploads/2013/07/img_4566.jpgIMG_4566https://aylo6061.com/wp-content/uploads/2013/07/img_4561.jpgIMG_45612016-07-23T03:18:23+00:00monthlyhttps://aylo6061.com/2013/07/25/an-electrophresis-system-for-diybio/https://aylo6061.com/wp-content/uploads/2013/07/img_4627.jpgIMG_4627https://aylo6061.com/wp-content/uploads/2013/07/img_4613.jpgIMG_4613https://aylo6061.com/wp-content/uploads/2013/07/illuminator.pngilluminatorBlue LED matrixhttps://aylo6061.com/wp-content/uploads/2013/07/pearl-blue-transilluminator.jpgPearl-Blue-TransilluminatorPearl Biotech transluminator2016-07-23T03:18:16+00:00monthlyhttps://aylo6061.com/2013/07/25/gelis-the-box/https://aylo6061.com/wp-content/uploads/2013/07/img_4630.jpgIMG_4630GelIS yet?2016-07-23T03:18:10+00:00monthlyhttps://aylo6061.com/2013/08/03/the-guide-to-diy-gel-illumination/https://aylo6061.com/wp-content/uploads/2013/08/img_4710.jpgIMG_4710Hmm. Didn't work!https://aylo6061.com/wp-content/uploads/2013/08/img_4727.jpgIMG_4727notice the arc of light in the diffuserhttps://aylo6061.com/wp-content/uploads/2013/08/img_4713.jpgIMG_4713Toplit Gel. This works suprisingly well, and gives you a lot of bang for your buck. Lots of reflections though.https://aylo6061.com/wp-content/uploads/2013/08/img_4705.jpgIMG_4705Blurry, but that is my fault. Definitely enough light for a photo.https://aylo6061.com/wp-content/uploads/2013/08/img_4703.jpgIMG_4703That's no cell phone, its a transluminator!https://aylo6061.com/wp-content/uploads/2013/08/img_4697.jpgIMG_46973x5 oriented the same way as above picture under diffuser. Note top to bottom of image drop off in intensity.https://aylo6061.com/wp-content/uploads/2013/08/img_4696.jpgIMG_46965x3 showing how hard it is to see the over-lit portions.https://aylo6061.com/wp-content/uploads/2013/08/img_4689.jpgIMG_4689aaaah, uneven!https://aylo6061.com/wp-content/uploads/2013/08/img_4678.jpgIMG_4678Really bright, and reflects a lot. This is the best shot I got of front-on illuminaiton.https://aylo6061.com/wp-content/uploads/2013/08/img_4668.jpgIMG_4668Diffused with translucent plastic, notice vignetting on top and right edges.2016-07-23T03:18:03+00:00monthlyhttps://aylo6061.com/2013/08/04/2nd-gelis-prototype/https://aylo6061.com/wp-content/uploads/2013/08/gel-comb.jpggel combThe least exciting parthttps://aylo6061.com/wp-content/uploads/2013/08/idea-note_20130803_234300_02.jpgIdea note_20130803_234300_02laser taper is caused by the beam spreading after focusinghttps://aylo6061.com/wp-content/uploads/2013/08/img_4767.jpgIMG_4767#forgotthecombatthelab? the box, and two dams for pouring the gel.https://aylo6061.com/wp-content/uploads/2013/08/idea-note_20130803_234300_01.jpgIdea note_20130803_234300_01This shape brought to you by cartoon mouse doors.https://aylo6061.com/wp-content/uploads/2013/08/img_4789.jpgIMG_4789Here is a top viewhttps://aylo6061.com/wp-content/uploads/2013/08/img_4793.jpgIMG_4793The second prototype! Fingers crossed, should be done in one more revision.2016-07-23T03:17:36+00:00monthlyhttps://aylo6061.com/2013/08/04/gelis-illuminator/https://aylo6061.com/wp-content/uploads/2013/08/assemblyfig.gifassemblyfigTransluminator...ASSEMBLE!https://aylo6061.com/wp-content/uploads/2013/08/img_4744.jpgIMG_4744Huge transluminator2016-07-23T03:17:25+00:00monthlyhttps://aylo6061.com/2013/08/04/gelis-design-thoughts-cutting-costs/https://aylo6061.com/wp-content/uploads/2013/08/img_4577.jpgIMG_4577Prototype 02016-07-23T03:17:19+00:00monthlyhttps://aylo6061.com/2013/08/04/3d-printed-microscope-adaptor/https://aylo6061.com/wp-content/uploads/2013/08/20130802_014658.jpg20130802_014658https://aylo6061.com/wp-content/uploads/2013/08/20130802_014630.jpg20130802_014630https://aylo6061.com/wp-content/uploads/2013/08/20130802_000100.jpg20130802_000100Tore these little curlies off with the flush cutter.https://aylo6061.com/wp-content/uploads/2013/08/20130802_000045.jpg20130802_000045YANMA Yet another microscope adapter, but at least its not for an iphone2016-07-23T03:17:11+00:00monthlyhttps://aylo6061.com/2013/08/08/dna-sequence-allignment-from-forward-and-reverse-reads/https://aylo6061.com/wp-content/uploads/2013/08/woohoo.pngwoohooX axis: number of base pairs overlapped from forward and reverse sequence Blue: number of pairs matching Green: "longest run"2016-07-23T03:17:04+00:00monthlyhttps://aylo6061.com/2013/08/10/gelis-second-cut/2016-07-23T03:16:56+00:00monthlyhttps://aylo6061.com/2013/08/10/gelis-prototype-two-fabrication-notes/https://aylo6061.com/wp-content/uploads/2013/08/img_4795.jpgIMG_4795left, new box. Right, old box2016-07-23T03:16:49+00:00monthlyhttps://aylo6061.com/2013/08/10/gelis-prototype-two-wet-test-success/https://aylo6061.com/wp-content/uploads/2013/08/img_48891.jpgIMG_4889https://aylo6061.com/wp-content/uploads/2013/08/img_48811.jpgIMG_4881Gel imaging is hard.https://aylo6061.com/wp-content/uploads/2013/08/img_48711.jpgIMG_4871https://aylo6061.com/wp-content/uploads/2013/08/img_48701.jpgIMG_4870Here is a closeup of the gel and the electrodes.https://aylo6061.com/wp-content/uploads/2013/08/img_48671.jpgIMG_4867https://aylo6061.com/wp-content/uploads/2013/08/img_48661.jpgIMG_4866Spot the difference!https://aylo6061.com/wp-content/uploads/2013/08/img_48541.jpgIMG_4854Pouring the gel, 1% Agarose in 1X TBE with gelgreen 5ul/mlhttps://aylo6061.com/wp-content/uploads/2013/08/img_4834.jpgIMG_4834https://aylo6061.com/wp-content/uploads/2013/08/img_4828.jpgIMG_4828https://aylo6061.com/wp-content/uploads/2013/08/img_48241.jpgIMG_48242016-07-23T03:16:42+00:00monthlyhttps://aylo6061.com/2013/08/14/microcentrifuge-tube-dimensions/2016-07-23T03:16:32+00:00monthlyhttps://aylo6061.com/2013/08/17/gelis-light-at-the-end-of-the-tunnel/https://aylo6061.com/wp-content/uploads/2013/08/img_4911.jpgIMG_4911GelIS Tray Holds Water2016-07-23T03:15:52+00:00monthlyhttps://aylo6061.com/2014/03/23/internet-enabled-incubator-progress/https://aylo6061.com/wp-content/uploads/2014/03/cool_incubator.pngcool_incubatorCooling takes a lot longer than heating because heating gets an extra heating term from resistive heatinghttps://aylo6061.com/wp-content/uploads/2014/03/heat_incubator.pngheat_incubatorThe incubator heating up and cooling down. Cooling during heating cycle is after the power was switched off.https://aylo6061.com/wp-content/uploads/2014/03/barebones.jpgbarebonesquick and dirty server with inputs for lighting and temperaturehttps://aylo6061.com/wp-content/uploads/2014/03/img_4901-e1395550343623.jpgIMG_4901The incubator is hanging out on my floor2016-07-23T03:15:32+00:00monthlyhttps://aylo6061.com/2013/09/20/gelis-simplified/https://aylo6061.com/wp-content/uploads/2013/09/img_1401.jpgIMG_1401https://aylo6061.com/wp-content/uploads/2013/09/img_1396.jpgprototype 4The latest GelIS prototype2016-07-23T03:15:21+00:00monthlyhttps://aylo6061.com/2013/10/01/gelis-final-cut/https://aylo6061.com/wp-content/uploads/2013/10/img_2005.jpgIMG_2005Test Runhttps://aylo6061.com/wp-content/uploads/2013/10/img_1991.jpgIMG_1991GelIS- Final Prototype2016-07-23T03:15:12+00:00monthlyhttps://aylo6061.com/2013/12/03/gelis-production-pt-1-lead-times/https://aylo6061.com/wp-content/uploads/2013/12/img_2127.jpgIMG_2127DC-DC converter, the heart of the Gel Power Supply2016-07-23T03:15:05+00:00monthlyhttps://aylo6061.com/2014/01/16/gelis-production-pt-2-laser-cutting-time/https://aylo6061.com/wp-content/uploads/2014/01/cutsheets.pngcutsheetshttps://aylo6061.com/wp-content/uploads/2013/12/img_2123.jpgIMG_2123parts for future gel boxes!https://aylo6061.com/wp-content/uploads/2013/12/img_2122.jpgIMG_2122https://aylo6061.com/wp-content/uploads/2013/12/img_2121.jpgIMG_21212016-07-23T03:14:58+00:00monthlyhttps://aylo6061.com/2014/01/16/allison-mine-day-trip/https://aylo6061.com/wp-content/uploads/2014/01/33-img_2806.jpg33-IMG_2806https://aylo6061.com/wp-content/uploads/2014/01/95-img_3281.jpg95-IMG_3281https://aylo6061.com/wp-content/uploads/2014/01/84-img_3212.jpg84-IMG_3212https://aylo6061.com/wp-content/uploads/2014/01/55-img_3014.jpg55-IMG_3014https://aylo6061.com/wp-content/uploads/2014/01/39-img_2879.jpg39-IMG_2879https://aylo6061.com/wp-content/uploads/2014/01/31-img_2799.jpg31-IMG_2799https://aylo6061.com/wp-content/uploads/2014/01/28-img_2792.jpg28-IMG_2792https://aylo6061.com/wp-content/uploads/2014/01/16-img_2724.jpg16-IMG_2724https://aylo6061.com/wp-content/uploads/2014/01/04-img_2634.jpg04-IMG_26342016-07-23T03:14:48+00:00monthlyhttps://aylo6061.com/2014/01/16/ae-1-camera-battery-adapter/https://aylo6061.com/wp-content/uploads/2013/10/img_1488.jpgIMG_1488Battery adaptor2016-07-23T03:14:41+00:00monthlyhttps://aylo6061.com/2014/03/20/sketching-supply-holster/https://aylo6061.com/wp-content/uploads/2014/03/img_4878.jpgIMG_4878https://aylo6061.com/wp-content/uploads/2014/03/img_4874.jpgIMG_4874https://aylo6061.com/wp-content/uploads/2014/03/img_4873.jpgIMG_4873Final model, showing pockets for scissors and rulerhttps://aylo6061.com/wp-content/uploads/2014/03/img_4870.jpgIMG_4870https://aylo6061.com/wp-content/uploads/2014/03/img_4868.jpgIMG_4868https://aylo6061.com/wp-content/uploads/2014/03/img_4865.jpgIMG_48652016-07-23T03:14:32+00:00monthlyhttps://aylo6061.com/2014/03/20/thing-o-matic-design-notes/https://aylo6061.com/wp-content/uploads/2014/03/img_4898.jpgIMG_4898https://aylo6061.com/wp-content/uploads/2014/03/img_4893.jpgIMG_4893Improper "set screw" configurationhttps://aylo6061.com/wp-content/uploads/2014/03/img_4890.jpgIMG_4890https://aylo6061.com/wp-content/uploads/2014/03/img_4888.jpgIMG_4888https://aylo6061.com/wp-content/uploads/2014/03/img_4886.jpgIMG_4886https://aylo6061.com/wp-content/uploads/2014/03/img_4879.jpgIMG_4879Ye Olde Thing O Matic2016-07-23T03:14:22+00:00monthlyhttps://aylo6061.com/2014/03/24/libv4l2-error-allocation-conversion-buffer-using-opencv-on-a-pi/2016-07-23T03:14:05+00:00monthlyhttps://aylo6061.com/2014/03/28/refrigeubator/https://aylo6061.com/wp-content/uploads/2014/03/img_4902.jpgIMG_4902https://aylo6061.com/wp-content/uploads/2014/03/img_4916.jpgIMG_4916https://aylo6061.com/wp-content/uploads/2014/03/img_4917.jpgIMG_4917https://aylo6061.com/wp-content/uploads/2014/03/img_4913.jpgIMG_4913https://aylo6061.com/wp-content/uploads/2014/03/img_4906.jpgIMG_4906OJ-SH105DM relay bank2016-07-23T03:13:54+00:00monthlyhttps://aylo6061.com/2014/04/05/verified-working-lightbulb-pcr/https://aylo6061.com/wp-content/uploads/2014/04/img_4936.jpgIMG_4936https://aylo6061.com/wp-content/uploads/2014/04/img_4953.jpgIMG_4953L to R: ladder, neg control, neg control, positive, positive, ladder (diff conc)2016-07-23T03:13:47+00:00monthlyhttps://aylo6061.com/2014/03/26/celery-causing-out-of-memory-with-opencv/2016-07-23T03:13:35+00:00monthlyhttps://aylo6061.com/2014/04/05/lightbulb-pcr-build-documentation/https://aylo6061.com/wp-content/uploads/2014/04/sketch7105850.jpgSketch7105850https://aylo6061.com/wp-content/uploads/2014/04/sketch7104913.jpgSketch7104913Exploded/Cross sectioned view of Lightbulb PCR Machinehttps://aylo6061.com/wp-content/uploads/2014/04/sketch711526.jpgSketch711526https://aylo6061.com/wp-content/uploads/2014/04/tumblr_mk7ahfqvyp1qaoxg6o5_1280.jpgOLYMPUS DIGITAL CAMERAOn a wire test tube rack at BOSSLABhttps://aylo6061.com/wp-content/uploads/2014/04/img_4974.jpgIMG_4974https://aylo6061.com/wp-content/uploads/2014/04/img_4973.jpgIMG_4973https://aylo6061.com/wp-content/uploads/2014/04/img_4970.jpgIMG_4970at30ts750 free soldered to wires.https://aylo6061.com/wp-content/uploads/2014/04/img_4969.jpgIMG_4969https://aylo6061.com/wp-content/uploads/2014/04/img_4968.jpgIMG_4968Really poorly connected relays are a holdover from old buildhttps://aylo6061.com/wp-content/uploads/2014/04/img_4966.jpgIMG_4966Recycling!2016-07-23T03:13:08+00:00monthlyhttps://aylo6061.com/2014/04/07/lightbulb-pcr-usage/https://aylo6061.com/wp-content/uploads/2014/04/img_4943.jpgIMG_4943Note the plug2016-07-23T03:13:02+00:00monthlyhttps://aylo6061.com/2014/04/13/olympus-xa-teardown-and-rebuild/https://aylo6061.com/wp-content/uploads/2014/02/img_4244.jpgIMG_4244https://aylo6061.com/wp-content/uploads/2014/02/img_4240.jpgIMG_4240https://aylo6061.com/wp-content/uploads/2014/02/img_4211.jpgXA uncoveredThe Olympus XA with several panels removedhttps://aylo6061.com/wp-content/uploads/2014/02/img_4210.jpgIMG_4210https://aylo6061.com/wp-content/uploads/2014/02/img_4207.jpgIMG_4207https://aylo6061.com/wp-content/uploads/2014/02/img_4206.jpgIMG_4206https://aylo6061.com/wp-content/uploads/2014/02/img_4200.jpgIMG_4200https://aylo6061.com/wp-content/uploads/2014/02/img_4198.jpgIMG_4198https://aylo6061.com/wp-content/uploads/2014/02/img_4196.jpgIMG_41962021-11-15T03:48:11+00:00monthlyhttps://aylo6061.com/2014/04/25/thermal-camera-progress/https://aylo6061.com/wp-content/uploads/2014/04/img_5356.jpgIMG_5356Much like a puch card reader, I was the interface between paper and comptuerhttps://aylo6061.com/wp-content/uploads/2014/04/i2cfail.jpgi2cfailMore like i2slow motion2016-07-23T03:12:35+00:00monthlyhttps://aylo6061.com/2014/04/14/gelis-redesign-dirty-gel-power-supply/https://aylo6061.com/wp-content/uploads/2014/04/wp-1397468052270.jpgwp-1397468052270New vs Old GelIS sketches2016-07-23T03:12:22+00:00monthlyhttps://aylo6061.com/2014/04/14/gelis-redesign-dirty-power-supply-part-ii/https://aylo6061.com/wp-content/uploads/2014/04/20140414_054526.jpg20140414_054526The test-desk. Power supply hooked up to an old gel tray with TBE in it.2016-07-23T03:12:14+00:00monthlyhttps://aylo6061.com/2014/04/17/scrolling-led-display/https://aylo6061.com/wp-content/uploads/2014/04/img_5233-e1397766121360.jpgIMG_5233flckr-less. Showing off some of the colors with a silly test patternhttps://aylo6061.com/wp-content/uploads/2014/04/sketch17413344.jpgSketch17413344The backside of the board.https://aylo6061.com/wp-content/uploads/2014/04/sketch17414313.jpgSketch17414313now each bit of a 64 bit row is addressable individuallyhttps://aylo6061.com/wp-content/uploads/2014/04/sketch174135611.jpgSketch174135611Each byte contains four pixelshttps://aylo6061.com/wp-content/uploads/2014/04/img_5232.jpgIMG_5232Wires. Everywhere.https://aylo6061.com/wp-content/uploads/2014/04/img_5229.jpgIMG_5229https://aylo6061.com/wp-content/uploads/2014/04/img_5227.jpgIMG_5227Ghosting is noticeable between rows here in this picutre. *(cylon noises)*https://aylo6061.com/wp-content/uploads/2014/04/img_5230.jpgIMG_5230They look pretty good...2016-07-23T03:12:04+00:00monthlyhttps://aylo6061.com/2014/04/23/atmega328p-assembly-i2c-transactions/https://aylo6061.com/wp-content/uploads/2014/04/i2ccap.jpgi2ccapReading 0x80. This is what the transaction looks like.2016-11-12T15:44:25+00:00monthlyhttps://aylo6061.com/2014/04/27/interrupt-driven-i2c-assembly-on-the-atmega328p/2016-07-23T03:11:36+00:00monthlyhttps://aylo6061.com/2014/04/28/thermal-camera-progress-still-images/2016-07-23T03:11:28+00:00monthlyhttps://aylo6061.com/2014/05/02/thermal-camera-live-view/2016-07-23T03:11:19+00:00monthlyhttps://aylo6061.com/2014/05/03/another-bloggable-debug-session-token-too-large-exceeds-yylmax/2016-07-23T03:10:51+00:00monthlyhttps://aylo6061.com/2014/05/11/awesome-grant-one-year-later/https://aylo6061.com/wp-content/uploads/2014/05/img_5478.jpgIMG_54782016-07-23T03:10:45+00:00monthlyhttps://aylo6061.com/2014/05/12/bluegene-so-far/https://aylo6061.com/wp-content/uploads/2014/05/plasmap_pslh8.jpgplasmap_pslh8pSHL8 is not the one I havehttps://aylo6061.com/wp-content/uploads/2014/05/bluegene-post.jpgbluegene postDeletion of stop codon2016-07-23T03:10:36+00:00monthlyhttps://aylo6061.com/2014/05/22/scrolling-neopixel-leds/https://aylo6061.com/wp-content/uploads/2014/05/the-letter-h.jpgthe letter hThis post brought to you by the letter H, and the dev board Tessel2020-02-04T07:00:56+00:00monthlyhttps://aylo6061.com/2014/09/30/kodak-ultramax-400-vs-fuji-superia-400/https://aylo6061.com/wp-content/uploads/2014/08/kodak-800-1.jpgkodak 800 1Kodakhttps://aylo6061.com/wp-content/uploads/2014/08/fuji-800.jpgfuji 800We were going 30 mph in a snow storm and this turned out ok on the fujihttps://aylo6061.com/wp-content/uploads/2014/08/fuji-800-2.jpgfuji 800 2Dark and cool, shot super fast and sneaky-likehttps://aylo6061.com/wp-content/uploads/2014/08/fuji-800-1.jpgfuji 800 1Dark, cool, fujihttps://aylo6061.com/wp-content/uploads/2014/08/kodak-800.jpgkodak 800Kinda grainyhttps://aylo6061.com/wp-content/uploads/2014/08/fuji-outside.jpgfuji outsideFuji Outside.https://aylo6061.com/wp-content/uploads/2014/08/fuji-outside-1.jpgfuji outside 1Woah sky! Fuji here.https://aylo6061.com/wp-content/uploads/2014/08/kodak-outside.jpgkodak outsideOutside Kodak.https://aylo6061.com/wp-content/uploads/2014/08/kodak-outside-2.jpgkodak outside 2Overexposed (night time) Kodak. Crazy Colors!https://aylo6061.com/wp-content/uploads/2014/08/kodak-inside-1.jpgkodak inside 1Kodak again.2016-07-23T03:10:19+00:00monthlyhttps://aylo6061.com/2015/05/30/bluegene-amplification-of-gblock-fragment/https://aylo6061.com/wp-content/uploads/2015/05/img_6241.jpgIMG_6241https://aylo6061.com/wp-content/uploads/2015/05/img_6239.jpgIMG_6239https://aylo6061.com/wp-content/uploads/2015/05/pcr-gel.jpgpcr gel2016-07-23T03:10:11+00:00monthlyhttps://aylo6061.com/2015/06/02/bluegene-a-restriction-enzyme-fail/https://aylo6061.com/wp-content/uploads/2015/06/gel-rd-analysis.jpggel rd analysislanes numbered from right to left. 2% agarose gel, with safe gel stain. lane 7 contains pUC19 and lane 8 and 9 contain samples that should be digested2016-07-23T03:10:02+00:00monthlyhttps://aylo6061.com/2015/06/02/bluegene-the-re-pcring-and-re-digesting/https://aylo6061.com/wp-content/uploads/2015/06/pcr-gel-ii-crop.jpgpcr gel ii crophttps://aylo6061.com/wp-content/uploads/2015/06/20150601_223227.jpg20150601_2232272016-07-23T03:09:37+00:00monthlyhttps://aylo6061.com/2015/06/10/16s-rrna-colony-pcr-the-orange-mystery-bacteria/https://aylo6061.com/wp-content/uploads/2015/06/16s-pcr-gel.jpg16s pcr gelhttps://aylo6061.com/wp-content/uploads/2015/06/16s-pcr-crop.jpg16s pcr crophttps://aylo6061.com/wp-content/uploads/2015/06/img_6407-e1433991798741.jpgIMG_6407Orange Mystery Bacteria2016-07-23T03:09:30+00:00monthlyhttps://aylo6061.com/2015/09/16/kossel-like-parallel-delta-robot-kinematics/https://aylo6061.com/wp-content/uploads/2015/09/print.pngRunning some sample G code in the simulatorhttps://aylo6061.com/wp-content/uploads/2015/09/delta-2.pngdelta 2https://aylo6061.com/wp-content/uploads/2015/09/delta.pngDeltaA pretty weird delta2016-07-23T03:09:22+00:00monthlyhttps://aylo6061.com/2016/01/09/misfit-flash-teardown/https://aylo6061.com/wp-content/uploads/2016/01/img_20160107_175215655.jpgIMG_20160107_175215655https://aylo6061.com/wp-content/uploads/2016/01/img_20160107_180357155.jpgIMG_20160107_180357155https://aylo6061.com/wp-content/uploads/2016/01/img_20160107_180634815.jpgIMG_20160107_180634815https://aylo6061.com/wp-content/uploads/2016/01/img_20160107_180747789.jpgIMG_20160107_180747789https://aylo6061.com/wp-content/uploads/2016/01/rf-nuggets.jpgrf nuggetshttps://aylo6061.com/wp-content/uploads/2016/01/img_20160107_182709479.jpgIMG_20160107_182709479https://aylo6061.com/wp-content/uploads/2016/01/img_20160107_182526599.jpgIMG_20160107_182526599https://aylo6061.com/wp-content/uploads/2016/01/leds-on1.jpgleds onhttps://aylo6061.com/wp-content/uploads/2016/01/spi.jpgspihttps://aylo6061.com/wp-content/uploads/2016/01/tagconnect.jpgtagconnect2016-07-29T08:25:06+00:00monthlyhttps://aylo6061.com/2016/01/09/re-flashing-the-misfit-flash/https://aylo6061.com/wp-content/uploads/2016/01/img_20160107_2008155311.jpgIMG_20160107_200815531https://aylo6061.com/wp-content/uploads/2016/01/selection_016.pngSelection_016https://aylo6061.com/wp-content/uploads/2016/01/all-your-leds.pngall your ledshttps://aylo6061.com/wp-content/uploads/2016/01/flash-banks.pngflash bankshttps://aylo6061.com/wp-content/uploads/2016/01/after-erase.pngafter erasehttps://aylo6061.com/wp-content/uploads/2016/01/program.pngprogramhttps://aylo6061.com/wp-content/uploads/2016/01/img_20160109_241816569.jpgIMG_20160109_241816569https://aylo6061.com/wp-content/uploads/2016/01/img_20160108_211731262.jpgIMG_20160108_2117312622016-07-23T03:09:00+00:00monthlyhttps://aylo6061.com/2016/01/13/kossel-adjustable-bed-conductive-probing/https://aylo6061.com/wp-content/uploads/2016/01/img_20160112_220954941.jpgIMG_20160112_220954941https://aylo6061.com/wp-content/uploads/2016/01/img_20160112_221301982.jpgIMG_20160112_221301982The print head on Screw Loosehttps://aylo6061.com/wp-content/uploads/2016/01/img_20160112_221859832.jpgIMG_20160112_221859832https://aylo6061.com/wp-content/uploads/2016/01/img_20160112_220928012.jpgIMG_20160112_220928012Bed adjuster, with thumb screws above and below the bed.https://aylo6061.com/wp-content/uploads/2016/01/img_20160112_221320785.jpgIMG_20160112_221320785I have no desire to add more wires to this rat nest.https://aylo6061.com/wp-content/uploads/2016/01/64.jpgDCIM100GOPROGOPR0064.DCIM100GOPROGOPR0064.https://aylo6061.com/wp-content/uploads/2016/01/delta-triangle1.pngdelta trianglePossible endpoints for a tower alpha, an arm of length L, and a tower distance D.https://aylo6061.com/wp-content/uploads/2016/01/delta-triangle.pngdelta triangleThe triangle created by the delta printer2016-07-23T03:08:44+00:00monthlyhttps://aylo6061.com/2016/01/13/gauging-kossel-z-accuracy/2016-07-23T03:08:23+00:00monthlyhttps://aylo6061.com/2016/01/15/putting-gopros-in-milling-machines/https://aylo6061.com/wp-content/uploads/2016/01/drawing.pngDRAWINGhttps://aylo6061.com/wp-content/uploads/2016/01/img_20160108_165622138.jpgIMG_20160108_165622138https://aylo6061.com/wp-content/uploads/2016/01/img_20160108_103216887.jpgIMG_20160108_103216887https://aylo6061.com/wp-content/uploads/2016/01/img_20160108_181540391.jpgIMG_20160108_181540391https://aylo6061.com/wp-content/uploads/2016/01/img_20160108_175839682.jpgIMG_20160108_1758396822016-07-23T03:08:08+00:00monthlyhttps://aylo6061.com/2016/01/15/the-kossel-science-project/https://aylo6061.com/wp-content/uploads/2015/05/img_6160.jpgIMG_6160https://aylo6061.com/wp-content/uploads/2015/05/img_6095.jpgIMG_6095https://aylo6061.com/wp-content/uploads/2015/05/img_6078.jpgIMG_60782016-07-23T03:07:49+00:00monthlyhttps://aylo6061.com/2016/01/21/commandeering-the-misfit-flash-board/https://aylo6061.com/wp-content/uploads/2016/01/button0.jpgbutton0https://aylo6061.com/wp-content/uploads/2016/01/setup-w-friend1.jpgsetup w friend1https://aylo6061.com/wp-content/uploads/2016/01/temps.pngtempshttps://aylo6061.com/wp-content/uploads/2016/01/flash-button-hold2.pngflash button hold2https://aylo6061.com/wp-content/uploads/2016/01/energizer_chart.pngenergizer_chart2016-08-30T04:13:02+00:00monthlyhttps://aylo6061.com/2016/02/11/gravity-app-for-misift-flash/https://aylo6061.com/wp-content/uploads/2016/02/atan1.pngatanhttps://aylo6061.com/wp-content/uploads/2016/02/atan.pngatanhttps://aylo6061.com/wp-content/uploads/2016/02/unit-circ-illus.pngunit circ illus2016-07-23T03:03:33+00:00monthlyhttps://aylo6061.com/2016/07/03/custom-service-with-sd110-the-nrf51288/https://aylo6061.com/wp-content/uploads/2016/07/screenshot_2016-07-03-15-10-42.pngScreenshot_2016-07-03-15-10-42https://aylo6061.com/wp-content/uploads/2016/07/g65681.pngg6568https://aylo6061.com/wp-content/uploads/2016/07/text4926-7.pngtext4926-7https://aylo6061.com/wp-content/uploads/2016/07/g6742.pngg6742https://aylo6061.com/wp-content/uploads/2016/07/path3459-1.pngpath3459-12016-07-23T03:01:39+00:00monthlyhttps://aylo6061.com/diybio-protocols-and-information/2014-04-08T00:54:57+00:00weekly0.6https://aylo6061.com/2013/07/19/my-chorded-keyboard-vision/https://aylo6061.com/wp-content/uploads/2013/07/desk.jpgdeskcrazy busy desk2013-07-19T15:21:29+00:00monthlyhttps://aylo6061.com/downloads/2014-06-05T01:02:18+00:00weekly0.6https://aylo6061.com/revobots/2012-04-24T01:22:51+00:00weekly0.6https://aylo6061.comdaily1.02025-07-23T03:43:40+00:00